249 specimens of Vanmanenia from the Yunnan of China were divided into 13 groups according to localities, 20 general characteristics were measured when analyzed by the methods of multivariate morphometrics. The results showed that the specimens from the different localities could be divided into 3 groups according to drainages, named Lancangjiang Group, Lixiangjiang Group and Yuanjiang Group. The coefficient of difference was more than critical value (1.28) among 3 groups. And there was no mediacy in the scatter plots by principal component analysis among 3 groups. So 3 groups should be given status of 3 different species. It was inferred that specimens in Lixiangjiang Group should be Vanmanenia tetraloba (Mai, 1978) according to the administrative subarea of Vietnam in original description, and specimens in Yuanjiang Group should be V. striata Chen (1980). Specimens in Lancangjiang Group were distinguished from V. tetraloba  and V. striata  by the combination of characteristics, including compressed body, large pectoral and pelvic fins, small skull. It was confirmed that the specimen of  Vanmanenia  from Zhangjiuhe (a branch of Jinshajiang) in Luquan County is not V. tetraloba  rather than V. pingchowensis after examined the one specimen kept in Kunming Institute of Zoology, the Chinese Academy of Sciences.