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| Genome-wide developed microsatellite markers for the melon thrips Thrips palmi Karny (Thysanoptera: Thripidae) |
| Yongfu Gao1, 2, Yajun Gong2, Ling Ma2, Lijun Cao2, Jincui Chen2, Min Chen1 *, Shujun Wei2 * |
| 1Beijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing 100083, China
2Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
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Abstract The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and polymorphism. These 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to 0.8490. The observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148, respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi.
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| Fund:This study was provided jointly by Promotion and Innovation of Beijing Academy of Agriculture and Forestry Sciences (KJCX20180113), the Natural Science Foundation of Beijing Municipality (6184037), and Beijing Key Laboratory of Environmentally Friendly Pest Management on Northern Fruits (BZ0432). |
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Corresponding Authors:
Min Chen, Shujun Wei
E-mail: minch@bjfu.edu.cn, shujun268@163.com
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2019, Vol. 44 
