Lihong Dang, Chengwen Li, Dongxue Wang, Yuxin Gao, Linpeng Zhao, Bin Zhang
预出版日期: 2024-03-15
Each species within the
order Thysanoptera has a distinct mitochondrial gene order displaying a high
level of gene rearrangement, particularly massive in Phlaeothripidae. To
understand the evolutionary patterns of rearrangement in this family, we
sequenced the complete mitochondrial genome of
Psephenothrips eriobotryae, a
notable potential
pest of loquat trees. Its mitogenome is a circular DNA molecule (15,413 bp in size) composed
of 37 genes: 13 PCGs, 22 tRNAs, two rRNAs, and two putative control regions (CRs).
By comparing with the ancestral mitogenome of arthropods,
P. eriobotryae exhibits notable gene rearrangement variations. However, it shares four
conserved gene blocks with three closely related species in Phlaeothripinae. In
the phylogenetic tree,
P. eriobotryae was clustered with these three leaf-feeding Phlaeothripinae species.
The presence of more similar conserved gene blocks probably
indicates a strong correlation between gene rearrangements and the phylogeny
within Phlaeothripidae. Furthermore, the mitogenome of
P. eriobotryae shows some characteristics: (a) two putative
CRs in which CR2 is the copy of partial CR1 with 99.62% sequence similarity, and CR1 consists of two 237 bp repeat
unites; (b) high A+T content of 82.6% in overall base composition; (c)
trnS1 lacking a complete dihydrouridine (DHU) arm; (d) the protein coding
gene,
atp8 started with TTG instead of the more common ATN, while
nad3 terminated by TAG rather than TAA; (e) translocations and inversions
observed in fifteen of the 37 genes.