Three new genera of the spider family Nesticidae, Hamus gen. nov., Nescina gen. nov. and Wraios gen. nov., are reported. Three new species, Hamus bowoensis sp. nov., Nescina minuta sp. nov. and Wraios longiembolus sp. nov., showing remarkable characters in palpal and epigynal morphology, are described based on specimens collected in Tibet and Yunnan, China. All the type specimens are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing.