A new freshwater turbellarian species, Dugesia sinensis Chen & Wang, sp. nov., is described, which was collected from Caoxi rivulet, Shaoguan, Guandong Province, China. The morphology, reproduction, histochemical localization of AChE and phylogenetics based on COI gene of the new species was observed and analysed. The new species differs from its sister species in the developed diagram of the male atrium, the bursal canal to the left of the copulatory apparatus and distance (1 mm) between copulatory bursa and the mouth. In addition, the results indicated that morphological differences of AChE+ nerve structure can be used as a distinguishable character for species identification of genera Dugesiidae.