The paper provides descriptions and illustrations of the internal reproductive systems in both sexes of a wide-spread species, Tipula (Yamatotipula) nova, and analyzes the possible usefulness of some internal reproductive structures for taxonomy in subgenus Tipula (Yamatotipula). The female ovipositor is described and illustrated for the first time. The original description of the male hypopygium is expanded upon the description of new morphological characters and detailed illustrations.