Three new species of the earthworm genus Amynthas Kinberg, 1867 (Clitellata: Oligochaeta: Megascolecidae) are described from southern China using an integrative taxonomic approach, which combines morphological traits and molecular phylogenetic analyses of the mitochondrial COI gene, namely Amynthas ganzhouensis Sun & Jiang, sp. nov. (Jiangxi), A. redactus Sun & Jiang, sp. nov. (Jiangxi, Guangdong, Guangxi, Hunan), and A. yixingensis Sun & Jiang, sp. nov. (Jiangsu, Jiangxi, Anhui). A. ganzhouensis Sun & Jiang, sp. nov. is assigned to the A. morrisi-group based on two pairs of spermathecal pores (sp) located at intersegments 5/6–6/7. A. redactus Sun & Jiang, sp. nov. is placed in the A. corticis-group, exhibiting four pairs of spermathecal pores at intersegments 5/6–8/9. A. yixingensis Sun & Jiang, sp. nov. exhibits intraspecific variation in the number and position of spermathecal pores: some populations conform to the A. sieboldi-group (three pairs of pores at 6/7–8/9), while others align with the A. corticis-group (four pairs at 5/6–8/9). Molecular analyses of the mitochondrial COI gene further confirm the distinct species status of these three taxa. These discoveries expand the known diversity of Amynthas in southern China.