The complete mitochondrial genome of the Gasteruption parvicollarium Enderlein (GenBank accession number: KR270643) was sequenced in the study. Totally 17 009 bp sequence was determined with an A+T content of 83.81%, including full set of typical animal mitochondrial genes. Two protein-coding and 10 tRNA genes as well as the A+T-rich region were rearranged compared with the putative ancestral arrangement of insects. Most of the rearranged genes were located in the ancestral region of trnI-trnQ-trnM-nad2-trnW-trnC-trnY- cox1-trnL2. The other rearranged genes are trnN and trnS1 located in the tRNA cluster trnA- trnR-trnN-trnS1-trnE-trnF and trnS2 located between cob and nad1. Remote inversion is dominant rearrangement event in G. parvicollarium mitochondrial genome, involving two protein-coding and 8 tRNA genes. Compared with the other mitochondrial genome reported in the same genus of Gasteruption, the inverted trnN was translocated to the tRNA cluster between cox1 and nad2 in G. parvicollarium. This is the first report of mitochondrial gene rearrangement occurred within genus of Hymenoptera. Our study points to a recently occurred gene rearrangement event in the Gasteruption species.