CHINESE
Zoological Systematics

Zoological Systematics >

2023 , Vol. 48 >Issue 3: 236 - 248

DOI: https://doi.org/10.11865/zs.2023301

Original Article

DNA barcoding for the identification of<em> Limonia </em>crane flies (Diptera: Limoniidae) from China, including a new species and a newly recorded species

  • Hanhuiying Lü ,
  • Yaru Chen ,
  • Jinlong Ren ,
  • Ning Wang ,
  • Ding Yang ,
  • Xiao Zhang
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  • 1Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine/College of Grassland Science, Qingdao Agricultural University, Qingdao, China 2Key Laboratory of Biohazard Monitoring and Green Prevention and Control in Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Huhhot, China 3Key Laboratory of the Pest Monitoring and Safety Control on the Crop and Forest, College of Agronomy, Xinjiang Agricultural University, Urumqi, China 4College of Plant Protection, China Agricultural University, Beijing, China

Online published: 2023-07-24

Supported by

This work was funded by the National Natural Science Foundation of China (32100356), the National Science & Technology Fundamental Resources Investigation Program of China (2019FY100400) and the National Key R&D Program of China (2022YFD1401102).

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Abstract

The genus Limonia Meigen, 1803 is globally distributed with 216 known species/subspecies, of which 30 are recorded from China. In this study, we firstly use DNA barcodes (mt COI) to identify specimens of Limonia from China, result in a discovery of three species. Considering the morphological data, these species were identified as L. albiterminalis Lü, Ren & Zhang, sp. nov., L. juvenca Alexander, 1935 (new record in China), and L. macrostigma (Schummel, 1829), respectively. Six COI sequences of the three species were provided, analyzed with other 24 COI sequences from Limonia species. The result indicates that intraspecific distances in the genus are generally less than 2.2%, interspecific distances range from 7.9% to 17.2%, and there is no overlap between intra- and interspecific distances. Redescription and illustrations of L. juvenca and illustration of male hypopygium of L. macrostigma, as well as the key to the Chinese Limonia crane flies, are all provided.

Cite this article

Hanhuiying Lü , Yaru Chen , Jinlong Ren , Ning Wang , Ding Yang , Xiao Zhang . DNA barcoding for the identification of<em> Limonia </em>crane flies (Diptera: Limoniidae) from China, including a new species and a newly recorded species[J]. Zoological Systematics, 2023 , 48(3) : 236 -248 . DOI: 10.11865/zs.2023301

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