Phylogenomic approaches in systematic studies

Junxia Zhang, Jiaxing Lai

Zoological Systematics ›› 2020, Vol. 45 ›› Issue (3) : 151-162.

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Zoological Systematics ›› 2020, Vol. 45 ›› Issue (3) : 151-162. DOI: 10.11865/zs.202021
Communication

Phylogenomic approaches in systematic studies

  • Junxia Zhang, Jiaxing Lai
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Phylogenomic approaches in systematic studies

  • Junxia Zhang, Jiaxing Lai
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摘要

Phylogenomic approaches are growingly applied in the systematic studies of animals with the rapid advancement of sequencing and analytical tools. Aiming to accelerate the systematic studies using genome-scale data in China, this paper outlines the commonly used techniques for generating phylogenomic datasets: RNA-seq based transcriptome, target-enrichment based UCEs and AHE, RAD-seq, whole genome sequencing, and briefly discusses the pros and cons of each technique. Major analytical procedures for the 1KITE transcriptome pipeline, the PHYLUCE (UCEs) and AHE pipelines and the PLWS (low-coverage whole genome sequencing) pipeline are summarized, and recent achievements valuable for phylogenomic analyses are introduced.

Abstract

Phylogenomic approaches are growingly applied in the systematic studies of animals with the rapid advancement of sequencing and analytical tools. Aiming to accelerate the systematic studies using genome-scale data in China, this paper outlines the commonly used techniques for generating phylogenomic datasets: RNA-seq based transcriptome, target-enrichment based UCEs and AHE, RAD-seq, whole genome sequencing, and briefly discusses the pros and cons of each technique. Major analytical procedures for the 1KITE transcriptome pipeline, the PHYLUCE (UCEs) and AHE pipelines and the PLWS (low-coverage whole genome sequencing) pipeline are summarized, and recent achievements valuable for phylogenomic analyses are introduced.

关键词

AHE / RAD-seq / WGS / Transcriptome / UCEs

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导出引用
Junxia Zhang, Jiaxing Lai. Phylogenomic approaches in systematic studies[J]. Zoological Systematics. 2020, 45(3): 151-162 https://doi.org/10.11865/zs.202021
Junxia Zhang, Jiaxing Lai. Phylogenomic approaches in systematic studies[J]. Zoological Systematics. 2020, 45(3): 151-162 https://doi.org/10.11865/zs.202021

基金

This work was supported by the Advanced Talents Incubation Program of the Hebei University to Junxia Zhang (521000981324).

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