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Four new genera in three spider families are reported, namely Langlibaitiao Lin & Li, gen. nov., of Dictynidae O. Pickard-Cambridge, 1871, with Lathys inaffecta Li, 2017 (♂♀, China: Guangxi) as the type species; Huoyanluo Lin & Li, gen. nov., of Macrobunidae Petrunkevitch, 1928, with Huoyanluo ruanxiaoqi Lin & Li, sp. nov. (♂, China: Yunnan) as the type species and Gushangzao Lin & Li, gen. nov. and Gyro Lin & Li, gen. nov., of Theridiidae Sundevall, 1833, with Gushangzao shiqian Lin & Li, sp. nov. (♂♀, China: Xizang) and Gyro zeppeli Lin & Li, sp. nov. (♀, China: Yunnan) as the type species, respectively. Further 31 new species in 19 spider families are reported, including Agelenidae C.L. Koch, 1837: Troglocoelotes doul Lin & Li, sp. nov. (♀, China: Guangxi) and T. ruanxiaowu Lin & Li, sp. nov. (♀, China: Guangxi); Dictynidae: Langlibaitiao zhangshun Lin & Li, sp. nov. (♂, China: Hainan); Hahniidae Bertkau, 1878: Hahnia zhuyifani Lin & Li, sp. nov. (♀, China: Yunnan); Liocranidae Simon, 1897: Platnick xintongi Lin & Li, sp. nov. (♂♀, China: Xizang); Macrobunidae: Funny yanqing Lin & Li, sp. nov. (♂♀, China: Xizang) and Huoyanluo zhangzezhongi Lin & Li, sp. nov. (♂, China: Yunnan); Mysmenidae Petrunkevitch, 1928: Phricotelus yangxiong Lin & Li, sp. nov. (♂♀, China: Chongqing); Nemesiidae Simon, 1889: Raveniola shixiu Lin, Wang & Li, sp. nov. (♀, China: Guangxi) and R. xiezhen Lin, Wang & Li, sp. nov. (♂♀, China: Yunnan); Nesticidae Simon,1894: Speleoticus sicet Lin & Li, sp. nov. (♂♀, China: Sichuan); Ochyroceratidae Fage, 1912: Speocera yiduoi Lin & Li, sp. nov. (♂, China: Guangdong); Oecobiidae Blackwall, 1862: Uroctea chenyui Lin & Li, sp. nov. (♂♀, China: Guangxi); Oonopidae Simon, 1890: Orchestina xiebao Lin & Li, sp. nov. (♀, China: Chongqing); Philodromidae Thorell, 1869: Apollophanes lujiani Lin & Li, sp. nov. (♂♀, China: Guangxi) and Psellonus dawanqu Lin & Li, sp. nov. (♂♀, China: Guangdong); Salticidae Blackwall, 1841: Coccorchestes spark Lin & Li, sp. nov. (♂♀, Indonesia, West Papua), Onomastus zhuwu Lin & Li, sp. nov. (♂♀, China: Chongqing) and Synagelides huangxin Lin & Li, sp. nov. (♂♀, China: Chongqing); Sparassidae Bertkau,1872: Thelcticopis chongzu Lin & Li, sp. nov. (♂♀, China: Yunnan); Symphytognathidae Hickman,1931: Kirinua zhengqi Lin & Li, sp. nov. (♀, China: Guangxi); Synotaxidae Simon,1895: Tekellina haosiwen Lin & Li, sp. nov. (♂♀, China: Chongqing) and T. huihangi Lin & Li, sp. nov. (♂♀, China: Zhejiang); Theridiidae: Chrosiothes pengqi Lin & Li, sp. nov. (♀, China: Chongqing), Coscinida hantao Lin & Li, sp. nov. (♂♀, China: Chongqing), Gushangzao goemon Lin & Li, sp. nov. (♂♀, Japan, Iriomotejima Island), Theridion chenzhangfui Lin & Li, sp. nov. (♂♀, China: Zhejiang) and Yaginumena xuanzan Lin & Li, sp. nov. (♂♀, China: Chongqing); Theridiosomatidae Simon, 1881: Coddingtonia chenyufengi Lin & Li, sp. nov. (♀, Vietnam: Hai Phong) and Sennin zhangxinae Lin & Li, sp. nov. (♂♀, Vietnam: Hai Phong); Zodariidae Thorell, 1881: Mallinella zhoushengboi Lin & Li, sp. nov. (♂♀, China: Chongqing). Four new combinations in three spider families are proposed: Philodromidae: Psellonus kianganensis (Barrion & Litsinger, 1995) comb. nov.; Theridiidae: Gushangzao pelorosus (Zhu, 1998) comb. nov. and Dictynidae: Langlibaitiao chishuiensis (Zhang, Yang & Zhang, 2009) comb. nov. and L. inaffectus (Li, 2017) comb. nov.